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Generally, yes absolutely. I’ve been doing a project called “NanoSavSeq” (Nanopore Saliva Sequencing) in my free time. It’s published on dat right now since the raw files for Nanopore are really big (got too big for hashbase). There is one company doing it as well, but my version is completely open source and I’ve optimized it for affordable automation.

To give you a sense, you can buy one for 1k and do as much detection as a 64k device, and it’s small enough to fit in a backpack. One device should be able to do 500-1000 tests per 24hrs at a cost of about $10 per test, not including labor.




Is this with multiplexing? Or are you extending the flowcell life?


Multiplexing. I use barcoded primers to amplify the sample, then pool and sequence


Would love to know more. This is fascinating.


The dat website is at dat://aaca379867bff648f454337f36a65c8239f2437538f2a4e0b4b5eb389ea0caff

You can visit with the beaker browser, or share it through dat so it won't ever go down.

You can also visit it at http://www.nanosavseq.com/ (DNS is not up yet, http://167.172.195.83/book/index.html is direct)

It's embarrassingly barren right now, mainly since I've encountered some big problems with getting my DNA quantifier out of storage to start doing a lot more experiments. I'm getting that on Tuesday, so will be updating site then.


The book / documentation is very clean and presented in a fantastic way. May I ask what engine you are using for presenting this book?


mdbook! By the folks making the Rust docs. I love their formatting.


Would you like to work together on this? This is very interesting stuff.


Would love to. Feel free to email me at koeng101<at>gmail.




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