Well, for most microorganisms, traditional genetic engineering is perfectly fine. You just create long homology ends and transform the cell and it works peachy keen. (Ironically, the one organism for which this spectacularly fails is E. coli, requiring the lambda-red system).
For 'higher eukarya' the big problem is you can still do the long homology ends, but a competing process is random insertion. Basically (if my understanding is correct), CRISPR reduces the competing process and makes specific insertion of DNA the dominant result. Sometimes, though, having multiple random insertion is not a huge problem.
For 'higher eukarya' the big problem is you can still do the long homology ends, but a competing process is random insertion. Basically (if my understanding is correct), CRISPR reduces the competing process and makes specific insertion of DNA the dominant result. Sometimes, though, having multiple random insertion is not a huge problem.